Catalogue number
CYT-903
Synonyms
Tumor Necrosis Factor, TNFA, Tumor Necrosis Factor Ligand
Superfamily Member 2, Cachectin, TNF-Alpha, TNFSF2, TNF-A, Tumor Necrosis Factor
(TNF Superfamily, Member 2), Tumor Necrosis Factor-Alpha, TNF,
Macrophage-Derived, TNF, Monocyte-Derived, TNF Superfamily, APC1 Protein,
Member 2, DIF, Tumor necrosis factor.
Introduction
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Description
TNF a produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 163 amino acids (77-233a.a.) and
having a molecular mass of 18.1kDa.
(Molecular size on SDS-PAGE will appear at
approximately 18-28kDa).
TNF a is expressed with a 6
amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
TNF a protein solution (1mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater than 90.0% as determined bySDS-PAGE.
Biological Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma
cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is ≤ 0.2 ng/ml.
Amino acid sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV
PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL
GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
