Catalogue number
CYT-208
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Introduction
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Description
Interleukin-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17000 Dalton.
The IL-1b is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 50mM Phosphate buffer pH=7.1 and 150mM NaCl.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Val-Arg-Ser.
Biological Activity
The specific activity as determined in the test of augmentation of lymphocyte proliferation assay using mouse thymus was found to be 200,000,000 IU/mg.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.631 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-1 beta Recombinant as a Reference Standard.
References
1.
Title: Proinflammatory Cytokine IL-1b Stimulates IL-8 Synthesis in Mast Cells via a Leukotriene B4 Receptor 2-Linked Pathway, Contributing to Angiogenesis.
Publication: Published online before print March 1, 2010, doi: 10.4049/ jimmunol.0901735 The Journal of Immunology April 1, 2010 vol. 184 no. 7 3946-3954.
Link: http://www.jimmunol.org/content/184/7/3946.full
Application: Level of IL-8 mRNA was dramatically enhanced by IL-1b,HMC-1 cells were stimulated with IL-1b.
2.Title:Contribution of IL-1 to resistance to Streptococcus pneumoniae infection .
Publication: Int. Immunol. (2008) 20 (9): 1139-1146. doi: 10.1093/intimm/dxn071 First published online: July 1, 2008
Link:http://intimm.oxfordjournals.org/content/20/9/1139.full
3.Title: Hydrogen sulphide decreases
IL-1b induced activation of fibroblast?like synoviocytes from patients with
osteoarthritis
Publications: journal of cellular and molecular medicine
19.1 (2015): 187-197
Link: http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12405/epdf
4. Title: Rifampin Augments Cytokine-Induced
Nitric Oxide Production in Human Alveolar Epithelial Cells
Publications: Antimicrobial agents and chemotherapy 50.1
(2006): 396-398
Link: http://aac.asm.org/content/50/1/396.full
5. Title: Adiponectin may contribute to synovitis
and joint destruction in rheumatoid arthritis by stimulating vascular endothelial
growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13
expression in fibroblast-like synoviocytes more than proinflammatory mediators
Publication: Arthritis Res Ther 11.6 (2009): R161.
Link: http://www.biomedcentral.com/content/pdf/ar2844.pdf
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
