Catalogue number
CYT-206
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Introduction
IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I IFNs.
Description
IFN-gamma Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 17kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 4.6.
Solubility
It is recommended to reconstitute the lyophilized IFN-gamma in sterile distilled water or 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized IFN gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI
VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS
VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ.
Biological Activity
The specific activity as determined in a viral resistance assay is < 0.05 ng/ml, corresponding to a specific activity of 2.0 x 10,000,000 IU/mg.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-g as a Reference Standard.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
References
1.Title:Candida albicans abrogates the expression of IFN-G-inducible protein-10 in human keratinocytes.
Publication:Article first published online: 18 JUL 2008DOI: 10.1111/j.1574-695X.2008.00457.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.2008.00457.x/full
2. Title: Rifampin Augments Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial Cells
Publications: Antimicrobial agents and chemotherapy 50.1 (2006): 396-398
Link: http://aac.asm.org/content/50/1/396.full
3.Title: Selective Activation of Human Dendritic Cells by OM-85 through a NF-kB and MAPK Dependent Pathway
Publications: PloS one 8.12 (2013): e82867
Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082867#pone-0082867-g005
4.Title: A New Strategy to Induce Effective Antitumour Response In Vitro and In Vivo
Publications: Scandinavian journal of immunology 68.3 (2008): 287-296
Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2008.02140.x/full
5.Title: Capture of Tumor Cell Membranes by Trogocytosis Facilitates Detection and Isolation of Tumor-Specific Functional CTLs
Publication: Cancer research 68.6 (2008): 2006-2013.
Link: http://cancerres.aacrjournals.org/content/68/6/2006.full
6. Title: Curcumin induces maturation-arrested dendritic cells that expand regulatory T cells in vitro and in vivo
Publication: Clinical & Experimental Immunology 162.3 (2010): 460-473.
Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2010.04232.x/full
