Catalogue number
PRO-786
Synonyms
Sorting nexin-5 isoform a, Sorting nexin-5, SNX5.
Introduction
Sorting nexin-5 (SNX5) belongs to the sorting nexin family, whose members contains a phox (PX) domain, (which is a phosphoinositide binding domain) and are involved in intracellular trafficking. SNX5 protein is a component of the mammalian retromer complex, which facilitates cargo recovery from endosomes to the trans-Golgi network. SNX5 binds to the Fanconi anemia, complementation group A protein.
Description
SNX5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 427 amino acids (1-404 a.a) and having a molecular mass of 49.2kDa.
SNX5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SNX5 protein solution (0.25mg/ml) in phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAVPEL LQQQEEDRSK LRSVSVDLNV DPSLQIDIPD ALSERDKVKF
TVHTKTTLPT FQSPEFSVTR QHEDFVWLHD TLIETTDYAG LIIPPAPTKP DFDGPREKMQ KLGEGEGSMT
KEEFAKMKQE LEAEYLAVFK KTVSSHEVFL QRLSSHPVLS KDRNFHVFLE YDQDLSVRRK NTKEMFGGFF
KSVVKSADEV LFTGVKEVDD FFEQEKNFLI NYYNRIKDSC VKADKMTRSH KNVADDYIHT AACLHSLALE
EPTVIKKYLL KVAELFEKLR KVEGRVSSDE DLKLTELLRY YMLNIEAAKD LLYRRTKALI DYENSNKALD
KARLKSKDVK LAEAHQQECC QKFEQLSESA KEELINFKRK RVAAFRKNLI EMSELEIKHA RNNVSLLQSC
IDLFKNN.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
