Catalogue number
PRO-2181
Synonyms
Fibronectin
Leucine Rich Transmembrane Protein 3, Fibronectin-Like Domain, Containing Leucine-Rich Transmembrane Protein 3, HH21, Leucine-Rich Repeat, Transmembrane Protein FLRT3,
KIAA1469.
Introduction
Fibronectin Leucine Rich Transmembrane Protein
3, also known as FLRT3 is a member of the fibronectin leucine rich
transmembrane protein (FLRT) family. FLRT3 contains one fibronectin type-III
domain as well as 10 LRR (leucine-rich) repeats and is expressed in the kidney,
brain, pancreas, skeletal muscle, lung, liver, placenta, and heart. In addition, the members of the FLRT family
play a role in cell adhesion as well as receptor signaling. FLRT3 has been
implicated in neurite outgrowth after nerve damage, as a positive regulator of
FGF signalling and in homotypic cell adhesion. Furthermore, FLRT3 has an essential
function in regulating cellular adhesion among the epithelial apical ridge and
the underlying mesenchyme and also in the establishment of the dorso-ventral
position of the ridge.
Description
FLRT3 produced in Sf9 insect cells is a single,
glycosylated polypeptide chain containing 508 amino acids (29-528a.a.) and
having a molecular mass of 57.6kDa. (Molecular size on SDS-PAGE will appear at
approximately 70-100kDa).
FLRT3 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Insect cells.
Physical Appearance
Sterile
filtered colorless solution.
Formulation
FLRT3
protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%
glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
KSCPSVCRCD AGFIYCNDRF LTSIPTGIPE DATTLYLQNN QINNAGIPSD
LKNLLKVERI YLYHNSLDEF PTNLPKYVKE LHLQENNIRT ITYDSLSKIP YLEELHLDDN SVSAVSIEEG
AFRDSNYLRL LFLSRNHLST IPWGLPRTIE ELRLDDNRIS TISSPSLQGL TSLKRLVLDG NLLNNHGLGD
KVFFNLVNLT ELSLVRNSLT AAPVNLPGTN LRKLYLQDNH INRVPPNAFS YLRQLYRLDM SNNNLSNLPQ
GIFDDLDNIT QLILRNNPWY CGCKMKWVRD WLQSLPVKVN VRGLMCQAPE KVRGMAIKDL NAELFDCKDS
GIVSTIQITT AIPNTVYPAQ GQWPAPVTKQ PDIKNPKLTK DHQTTGSPSR
KTITITVKSV TSDTIHISWK LALPMTALRL SWLKLGHSPA FGSITETIVT GERSEYLVTA LEPDSPYKVC MVPMETSNLY
LFDETPVCIE TETAPLRMYN PTTTLNREQE KEPYKNPNLP LEHHHHHH.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
