Catalogue number
ANT-632
Synonyms
Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42,
Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH,
NADP(+)-specific ICDH, IDP, PICD.
Type
Mouse Anti Human Monoclonal.
Introduction
Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
Clone
PAT25H10AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human IDH1 mAb, is
derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant human IDH1 protein 1-414 amino
acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.
Purification Method
IDH1 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western
blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity.
Since application varies, however, each investigation should be titrated by the
reagent to obtain optimal results.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
