Catalogue number
PRO-1646
Synonyms
R-spondin-3, Protein with TSP type-1 repeat, hPWTSR, Roof plate-specific spondin-3, hRspo3, Thrombospondin type-1 domain-containing protein 2, RSPO3, PWTSR, THSD2, THSD2, CRISTIN1.
Introduction
R-spondin-3 (RSPO3) belongs to the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein which is widely expressed in many tissues. RSPO3 contains 2 Furin-like repeats which have been found in various eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases, and one TSP type-1 domain. RSPO3 acts as an activator of the beta-catenin signaling cascade, initiating TCF-dependent gene activation.
Description
Recombinant Human R-Spondin-3 produced in HEK293 cells is a polypeptide chain starting at amino acid Gln at position 22 to amino acid Val at position 201, fused to an FC, 6 x His-tag at C-terminus, containing a total of 498 amino acids and having a Mw of 47.9 kDa. The protein migrates at 61kDa on SDS-PAGE.
RSPO3 is a truncated protein that lacks amino acid Gln at position 201 to amino acid H at position 272 and purified by proprietary chromatographic techniques.
Source
HEK293 cells.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
RSPO3 was lyophilized from a 0.2µm filtered solution in 20mM PB, and 150mM NaCl pH-7.2.
Solubility
It is recommended to quick spin followed by reconstitution of RSPO3 in PBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized RSPO3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RSPO3 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS PAGE.
Amino acid sequence
QNASRGRRQR RMHPNVSQGC QGGCATCSDY NGCLSCKPRL FFALERIGMK QIGVCLSSCP SGYYGTRYPD INKCTKCKAD CDTCFNKNFC TKCKSGFYLH LGKCLDNCPE GLEANNHTME CVSIVHCEVS EWNPWSPCTK KGKTCGFKRG TETRVREIIQ HPSAKGNLCP PTNETRKCTV DDIEGRMDEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
