Catalogue number
CYT-832
Synonyms
Angiopoietin-Like Protein 6, Angiopoietin-Related Protein 5, Angiopoietin-Related Growth Factor, Angiopoietin-Related Protein 6, AGF, ARP5.
Introduction
ANGPTL6 is a protein-coding gene which takes part is several processes. ANGPTL6 is involved in the wound healing process, promotes the chemotactic activity of endothelial cells and induce neovascularization. ANGPTL6 also counteracts high-fat diet-induced obesity and related insulin resistance through increased energy expenditure. Additionally ANGPTL6 promotes epidermal proliferation, remodeling and regeneration. Diseases linked with ANGPTL6 include enterobiasis, and gnathomiasis.
Description
ANGPTL6 Human Recombinant produced in E.coli is a polypeptide chain containing 460 amino acids and having a total molecular mass of 50.7 kDa. ANGPTL6 contains a N-Terminal 10 aa His-Tag.
Source
E.coli.
Physical Appearance
White lyophilized (freeze-dried) powder.
Formulation
Filtered (0.4 µm) and lyophilized from a 0.5mg/ml solution containing 0.05M Acetate buffer
pH 4.0.
Solubility
Add 0.1M acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10?g/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
Stability
Lyophilized ANGPTL6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ANGPTL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MKHHHHHHAS RAGAPRCTYT FVLPPQKFTG AVCWSGPAST RATPEAANAS ELAALRMRVG RHEELLRELQ RLAAADGAVA GEVRALRKES RGLSARLGQL RAQLQHEAGP GAGPGADLGA EPAAALALLG ERVLNASAEA QRAAARFHQL DVKFRELAQL VTQQSSLIAR LERLCPGGAG GQQQVLPPPP LVPVVPVRLV GSTSDTSRML DPAPEPQRDQ TQRQQEPMAS PMPAGHPAVP TKPVGPWQDC AEARQAGHEQ SGVYELRVGR HVVSVWCEQQ LEGGGWTVIQ RRQDGSVNFF TTWQHYKAGF GRPDGEYWLG LEPVYQLTSR GDHELLVLLE DWGGRGARAH YDGFSLEPES DHYRLRLGQY HGDAGDSLSW HNDKPFSTVD RDRDSYSGNC ALYQRGGWWY HACAHSNLNG VWHHGGHYRS RYQDGVYWAE FRGGAYSLRK AAMLIRPLKL
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
