Catalogue number
PRO-2242
Synonyms
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CD40.
Introduction
CD40 belongs to the TNF-receptor super family. CD40 has been found to be vital in mediating a wide range of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is accounted to coordinately regulate the expression of CD40 and its ligand, which is significant for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and functions as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be essential for amyloid-beta-induced microglial activation, and therefore is considered to be an early event in Alzheimer disease pathogenesis.
Description
CD40 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 180 amino acids (20-193 a.a.) and
having a molecular mass of 20.1kDa (Migrates at 18-28kDa on SDS-PAGE under
reducing conditions).
CD40 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
CD40 protein solution (1.0mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
LGQCVTCSDK
QYLHDGQCCD LCQPGSRLTS HCTALEKTQC HPCDSGEFSA QWNREIRCHQ HRHCEPNQGL RVKKEGTAES
DTVCTCKEGQ HCTSKDCEAC AQHTPCIPGF GVMEMATETT DTVCHPCPVG FFSNQSSLFE KCYPWTSCED
KNLEVLQKGT SQTNVICGLK SRMRHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
