Catalogue number
CYT-333
Synonyms
Osteogenic Protein 1, BMP-7.
Introduction
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.
Description
Bone Morphogenetic Protein-7 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, polypeptide chain containing 139 amino acids and having a molecular mass of 15679.97 Dalton.
The BMP-7 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.
Solubility
It is recommended to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in 20mM-100mM acetic acid at a concentration of 0.1-0.5mg per ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Stability
Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP 7 Human should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
References
1.Title: Pulpal and periapical response of dogs' teeth after pulpotomy and use of recombinant human bone morphogenetic protein-7 as a capping agent
Publications: J Dent Child 74 (2007): 79-84.
Link:
http://www.researchgate.net/profile/Francisco_Paula-Silva/publication/
5374974_Pulpal_and_periapical_response_of_dogs'_teeth_after_pulpotomy_
and_use_of_recombinant_human_bone_morphogenetic_protein-7_as_a_capping_agent/links/5413605e0cf2788c4b359755.pdf
2. Title: Pretreatment with Bone Morphogenetic Protein-7 (BMP-7) Mimics Ischemia
Preconditioning Following Intestinal Ischemia/Reperfusion Injury in The
Intestine and Liver
Publications: Shock (Augusta, Ga.)30.5 (2008): 532.
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800102/
3. Title: BMP-7 blocks mesenchymal conversion of
mesothelial cells and prevents peritoneal damage induced by dialysis fluid
exposure
Publication: Nephrology Dialysis Transplantation (2010): gfp618.
Link: http://ndt.oxfordjournals.org/content/early/2010/01/18/ndt.gfp618.full
