Catalogue number
PRO-029
Synonyms
CD32, CD32A, CDw32, FcGR, IGFR2, MGC23887, MGC30032, Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, Fc-gamma-RIIa, FcRII-a, FCG2, FCGR2A1.
Introduction
CD32a, also known as FCGR2A, belongs to a family of immunoglobulin Fc receptor genes located on the surface of various immune response cells. FCGR2A which is a cell surface receptor found on phagocytic cells like macrophages and neutrophils, takes part in the process of phagocytosis and clearing of immune complexes. FCGR2A initiates cellular responses against pathogens and soluble antigens by binding to IgG. FCGR2A, which is a low affinity receptor, binds to the Fc region of immunoglobulins gamma.
Description
FCGR2A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 208 amino acids (34-216 a.a) and having a molecular mass of 23.0kDa. FCGR2A is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
FCGR2A protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 50% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMQAAPP KAVLKLEPPW INVLQEDSVT LTCQGARSPE SDSIQWFHNG NLIPTHTQPS YRFKANNNDS GEYTCQTGQT SLSDPVHLTV LSEWLVLQTP HLEFQEGETI MLRCHSWKDK PLVKVTFFQN GKSQKFSRLD PTFSIPQANH SHSGDYHCTG NIGYTLFSSK PVTITVQVPS MGSSSPMG.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
