Catalogue number
PRO-2245
Synonyms
Platelet glycoprotein 4, Glycoprotein IIIb, GPIIIB, PAS IV, PAS-4, Platelet glycoprotein IV, GPIV, CD36, Cd36.
Introduction
Platelet
glycoprotein IV, also known as CD36 is an integral membrane glycoprotein which
has multiple physiological functions. CD36 is broadly expressed on a wide array
of cell types. CD36 is part of the scavenger receptor family and acts as a
multiligand pattern recognition receptor which interacts with a large number of
structurally dissimilar ligands. Furthermore, CD36 participates in lipid
metabolism and has been known as a fatty acid translocase which is required for
the binding
as well as transport of LCFA in cells & tissues. CD36 also takes a major part
in the initiation as well as pathogenesis of chronic inflammatory diseases such
as Alzheimer’s disease and atherosclerosis. CD36 is an integral membrane
protein which mainly functions as a receptor for thrombospondin & collagen.
CD36 directly mediates the cytoadherence of Plasmodium falciparum parasitized
erythrocytes.
Description
CD36 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 419 amino acids (30-439a.a.) and having a molecular mass of 47.4kDa (Molecular size on SDS-PAGE will appear at approximately 57-70kDa).
CD36 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Insect cells.
Physical Appearance
Sterile
filtered colorless solution.
Formulation
|
CD36 protein solution (1mg/ml)
contains phosphate buffered saline (pH7.4) and 10% glycerol.
|
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
ADPGDMLIEK TIKREVVLEE GTTAFKNWVK TGTTVYRQFW IFDVQNPDDV
AKNSSKIKVK QRGPYTYRVR YLAKENITQD PEDHTVSFVQ PNGAIFEPSL SVGTEDDNFT VLNLAVAAAP
HIYQNSFVQV VLNSLIKKSK SSMFQTRSLK ELLWGYKDPF LSLVPYPIST TVGVFYPYND TVDGVYKVFN
GKDNISKVAI IESYKGKRNL SYWPSYCDMI NGTDAASFPP FVEKSRTLRF FSSDICRSIY AVFGSEIDLK
GIPVYRFVLP ANAFASPLQN PDNHCFCTEK VISNNCTSYG VLDIGKCKEG KPVYISLPHF LHASPDVSEP
IEGLHPNEDE HRTYLDVEPI TGFTLQFAKR LQVNILVKPA RKIEALKNLK RPYIVPILWL NETGTIGDEK
AEMFKTQVTG KIKHHHHHH.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
