Catalogue number
CYT-034
Synonyms
Interferon alpha 2b, IFNA, INFA2, MGC125764, MGC125765.
Introduction
IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
Description
Interferon-a 2b Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. The Pegylated IFN-a 2b is produced by attaching a 20kDa mPEG-aldehyde to the N-terminal IFN alpha-2b.
Interferon-a 2b is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Colorless, clear and transparent solution.
Formulation
IFN-a 2b is supplied in solution (1.48mg/ml) containing 20mM Acetate Buffer (pH 6.0), 0.8%NaCl and 0.005% Polysorbate 80.
Stability
IFN alpha-2b PEG should be stored refrigerated at 2° to 8°C. Vials should be kept in their packaging to protect from light until the time of use.
Shaking and freezing should be avoided.
Purity
Greater than 97.0% as determined by SEC-HPLC.
Biological Activity
The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 3,000,000 IU/mg.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
