Catalogue number
PRO-300
Synonyms
MSS4, RASGFR3, RASGRF3, Rab-interacting factor.
Introduction
RABIF is a guanine-nucleotide-releasing protein is involved in vesicular transport. RABIF stimulates GTP-GDP exchange in Sec4 and Rab and binds to a subset of genetically related Rab proteins.
Description
RABIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids (1-123 a.a) and having a molecular mass of 16kDa.
RABIF is fused to a 20 amino acid His Tag at N-Terminus and purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear colorless solution.
Formulation
The RABIF protein solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MEPAEQPSELVSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV SHE.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
