Catalogue number
PRO-1895
Synonyms
docking protein 4, Downstream of tyrosine kinase 4, IRS-5, IRS5.
Introduction
Docking Protein 4(DOK4) is a
member of the DOK family. DOK proteins are enzymatically inert adaptor or
scaffolding proteins. The DOK proteins present a docking platform for the compilation
of multimolecular signaling complexes. DOK4 serves in RET-mediated neurite
outgrowth and has a positive role in activation of the MAP kinase pathway. DOK4
is a putative link with downstream effectors of RET in neuronal
differentiation. DOK4 is involved in the regulation of the immune response
induced by T-cells.
Description
DOK4 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 349 amino acids (1-326 a.a) and
having a molecular mass of 39.4kDa.DOK4 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
DOK4 protein solution (1mg/ml) contains 20mM Tris-HCl
buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMATNFSD IVKQGYVKMK SRKLGIYRRC WLVFRKSSSK GPQRLEKYPD
EKSVCLRGCP KVTEISNVKC VTRLPKETKR QAVAIIFTDD SARTFTCDSE LEAEEWYKTL SVECLGSRLN
DISLGEPDLL APGVQCEQTD RFNVFLLPCP NLDVYGECKL QITHENIYLW DIHNPRVKLV SWPLCSLRRY GRDATRFTFE AGRMCDAGEG
LYTFQTQEGE QIYQRVHSAT LAIAEQHKRV LLEMEKNVRL LNKGTEHYSY PCTPTTMLPR SAYWHHITGS
QNIAEASSYA GEGYGAAQAS SETDLLNRFI LLKPKPSQGD SSEAKTPSQ.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
