Catalogue number
PRO-1785
Synonyms
CAMP-Regulated Phosphoprotein 19kDa, ARPP-19, ARPP16, Endosulfine Alpha-Like, ARPP-16, ENSAL, CAMP-Regulated Phosphoprotein 19, Cyclic AMP Phosphoprotein 19 KD, ARPP19.
Introduction
cAMP-regulated phosphoprotein 19 (ARPP19) is protein phosphatase inhibitor which specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, ARPP19 specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, a vital condition to maintain cyclin-B1-CDK1 activity high during M phase. ARPP19 protein may indirectly boost GAP-43 expression. The 19 kDa cAMP-regulated phosphoprotein has a role in regulating mitosis by obstructing protein phosphatase-2A.
Description
ARPP19 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids (1-112 a.a) and having a molecular mass of 14.7kDa.
ARPP19 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ARPP19 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSAEVPE AASAEEQKEM EDKVTSPEKA EEAKLKARYP HLGQKPGGSD FLRKRLQKGQ KYFDSGDYNM AKAKMKNKQL PTAAPDKTEV TGDHIPTPQD LPQRKPSLVA SKLAG.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
