Catalogue number
HSP-059
Synonyms
Heat shock transcription factor, Y-linked, Heat shock transcription factor 2-like protein, HSF2-like, HSFY1, HSF2L, HSFY, HSFY2, Y-linked isoform 1, Y-Linked 1.
Introduction
Heat Shock Transcription Factor, Y-Linked 1 (HSFY1) is a member of the heat shock factor (HSF) family of transcriptional activators for heat shock proteins. HSFY1 is localizes to an area of chromosome Y which is from time to time deleted in infertile males and therefore is considered a gene for azoospermia. The genome has 2 identical duplicates of HSFY1 within a palindromic area.
Description
HSFY1 Human Recombinant produced in E. coli is a single polypeptide chain containing 424 amino acids
(1-401) and having a molecular mass of 47.5kDa.
HSFY1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The HSFY1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAHVSSE TQDVSPKDEL TASEASTRSP LCEHTFPGDS DLRSMIEEHA FQVLSQGSLL ESPSYTVCVS EPDKDDDFLS LNFPRKLWKI VESDQFKSIS WDENGTCIVI NEELFKKEIL ETKAPYRIFQ TDAIKSFVRQ LNLYGFSKIQ QNFQRSAFLA TFLSEEKESS VLSKLKFYYN PNFKRGYPQL LVRVKRRIGV KNASPISTLF NEDFNKKHFR AGANMENHNS ALAAEASEES LFSASKNLNM PLTRESSVRQ IIANSSVPIR SGFPPPSPST SVGPSEQIAT DQHAILNQLT TIHMHSHSTY MQARGHIVNF ITTTTSQYHI ISPLQNGYFG LTVEPSAVPT RYPLVSVNEA PYRNMLPAGN PWLQMPTIAD RSAAPHSRLA LQPSPLDKYH PNYN.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
