Catalogue number
PRO-1439
Synonyms
Cold shock domain-containing protein C2, RNA-binding
protein PIPPin, PIPPIN, DJ347H13.2.
Introduction
Cold shock domain-containing protein C2 (CSDC2) is RNA-binding factor that binds specifically to the very 3'-UTR ends of both histone H1 and H3. 3 mRNAs encompass the polyadenylation signal. The CSDPs (cold shock domain containing proteins) are one group of the evolutionarily conserved nucleic acid-binding proteins extensively distributed in bacteria, plants, animals, and involved in a variety of cellular processes, including adaptation to low temperature, cellular growth, nutrient stress and stationary phase. CSDC2 has a central role in the negative regulation of histone variant synthesis in the developing brain.
Description
CSDC2 Human Recombinant produced in E.coli is a single,
non-glycosylated polypeptide chain containing 176 amino acids (1-153) and
having a molecular mass of 19.2kDa. CSDC2 is fused to a 23 amino acid His-tag
at N-terminus.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The CSDC2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSMTSESTS
PPVVPPLHSP KSPVWPTFPF HREGSRVWER GGVPPRDLPS PLPTKRTRTY SATARASAGP VFKGVCKQFS
RSQGHGFITP ENGSEDIFVH VSDIEGEYVP VEGDEVTYKM CPIPPKNQKF QAVEVVLTQL APHTPHETWS
GQVVGS.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
