Catalogue number
PRO-2078
Synonyms
MYC Induced Nuclear Antigen, MINA53, MDIG, 60S Ribosomal
Protein L27a Histidine Hydroxylase, Mineral Dust-Induced Gene Protein, Histone
Lysine Demethylase MINA Ribosomal Oxygenase MINA, Nucleolar Protein 52, NO52, ROX,
Bifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase MINA, Myc-Induced
Nuclear Antigen, 53 KDa, Mineral Dust Induced Gene Protein, MYC-Induced Nuclear
Antigen, EC 1.14.11.-, Bifunctional lysine-specific demethylase and
histidyl-hydroxylase MINA.
Introduction
MYC Induced Nuclear Antigen, also known as MINA is an oxygenase which
can function both as a histone lysine demethylase and a ribosomal histidine hydroxylase. MINA is involved in the demethylation of trimethylated
Lys-9 on histone H3 (H3K9me3), leading to an increase in ribosomal RNA
expression. MINA also catalyzes the
hydroxylation of 60S ribosomal protein L27a on His-39. In addition, MINA plays a significant role in cell growth and
survival. MINA is implicated in ribosome
biogenesis, probably in the duration of the assembly process of pre-ribosomal
particles.
Description
MINA Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 485 amino acids (1-465 a.a) and
having a molecular mass of 54.9kDa.
MINA is fused to a 20 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
MINA protein solution (0.25mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH
MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK TETFFKEFWE QKPLLIQRDD PALATYYGSL
FKLTDLKSLC SRGMYYGRDV NVCRCVNGKK KVLNKDGKAH FLQLRKDFDQ KRATIQFHQP QRFKDELWRI
QEKLECYFGSLVGSNVYITP AGSQGLPPHY DDVEVFILQL EGEKHWRLYH PTVPLAREYS
VEAEERIGRP VHEFMLKPGD LLYFPRGTIH QADTPAGLAH STHVTISTYQ NNSWGDFLLD TISGLVFDTA
KEDVELRTGI PRQLLLQVES TTVATRRLSG FLRTLADRLE GTKELLSSDM KKDFIMHRLPPYSAGDGAEL STPGGKLPRL
DSVVRLQFKD HIVLTVLPDQ DQSDETQEKM VYIYHSLKNS RETHMMGNEE ETEFHGLRFP LSHLDALKQI WNSPAISVKD
LKLTTDEEKE SLVLSLWTEC LIQVV.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
