Catalogue number
PRO-1741
Synonyms
Fc Fragment Of IgE, High Affinity I, Receptor For; Alpha Polypeptide,
FCE1A, IgE Fc Receptor Subunit Alpha, FcERI, Fc-Epsilon RI-Alpha, Fc Epsilon RI
Alpha-Chain, Fc IgE Receptor, Alpha Polypeptide, High Affinity Immunoglobulin
Epsilon Receptor Alpha-Subunit, High Affinity Immunoglobulin Epsilon Receptor
Subunit Alpha, Immunoglobulin E Receptor, High-Affinity, Of Mast Cells, Alpha
Polypeptide.
Introduction
Fc
fragment of IgE, high affinity I, receptor for; alpha polypeptide (FCER1A)
binds to the Fc region of immunoglobulins epsilon. FCER1A is a high affinity
receptor. In addition FCER1A is responsible for starting the allergic response.
Binding of allergen to receptor-bound IgE leads to cell activation and the
release of mediators such as histamine which is responsible for the
manifestations of allergy. This receptor is contains of an alpha subunit, a
beta subunit, and two gamma subunits. FCER1A stands for the alpha subunit.
Among the diseases associated with FCER1A are mast-cell leukemia, and allergic
asthma.
Description
FCER1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (26-205aa) and having a molecular mass of 23.4kDa.
FCER1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
FCER1A protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSVPQKPKV SLNPPWNRIF KGENVTLTCN GNNFFEVSST KWFHNGSLSE
ETNSSLNIVN AKFEDSGEYK CQHQQVNESE PVYLEVFSDW LLLQASAEVV MEGQPLFLRC HGWRNWDVYK
VIYYKDGEAL KYWYENHNIS ITNATVEDSG TYYCTGKVWQ LDYESEPLNI TVIKAPREKY WLQ
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
