Catalogue number
PRO-2081
Synonyms
CREB/ATF BZIP Transcription Factor, Host Cell
Factor-Binding Transcription Factor Zhangfei, HCF-Binding Transcription Factor
Zhangfei, Zhangfei, ZF, SHP-Interacting Leucine Zipper Protein, SMILE, CREB/ATF
bZIP transcription factor.
Introduction
CREB/ATF BZIP Transcription Factor, also known as CREBZF activates
transcription strongly once bound to HCFC1. CREBZF suppresses the expression of
HSV proteins in cells infected with the virus in a HCFC1-dependent manner. CREBZF suppresses the HCFC1-dependent
transcriptional activation through CREB3 and reduces the quantity of CREB3 in
the cell. In addition, CREBZF is capable to down-regulate expression of a few
cellular genes in CREBZF-expressing cells.
Description
CREBZF Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 377 amino acids (1-354 a.a) and
having a molecular mass of 39.5kDa.
CREBZF is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
CREBZF protein solution (0.25mg/ml) containing PBS buffer
(pH 7.4), 20% glycerol,1mM DTT and 0.1mM PMSF.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMRHSLTK LLAASGSNSP TRSESPEPAA TCSLPSDLTR AAAGEEETAA
AGSPGRKQQF GDEGELEAGR GSRGGVAVRA PSPEEMEEEA IASLPGEETE DMDFLSGLEL ADLLDPRQPD
WHLDPGLSSP GPLSSSGGGS DSGGLWRGDD DDEAAAAEMQRFSDLLQRLL NGIGGCSSSS DSGSAEKRRR KSPGGGGGGG SGNDNNQAAT
KSPRKAAAAA ARLNRLKKKE YVMGLESRVR GLAAENQELR AENRELGKRV QALQEESRYL RAVLANETGL
ARLLSRLSGV GLRLTTSLFR DSPAGDHDYA LPVGKQKQDL LEEDDSAGGV CLHVDKDKVSVEFCSACARK ASSSLKM.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
