Catalogue number
PRO-2380
Synonyms
Inositol monophosphatase 3, IMP 3, IMPase 3, Golgi 3-prime
phosphoadenosine 5-prime phosphate 3-prime phosphatase, Golgi-resident PAP
phosphatase, gPAPP, Inositol monophosphatase domain-containing protein 1, Inositol-1(or
4)-monophosphatase 3, Myo-inositol monophosphatase A3.
Introduction
Inositol monophosphatase 3 (IMPAD1) belongs to the inositol monophosphatase family. IMPAD1 is restricted to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). IMPAD1 gene mutations cause the GRAPP type chondrodysplasia with joint dislocationss, and a pseudogene of the IMPAD1 gene is located on the long arm of chromosome 1.
Description
IMPAD1 Mouse Recombinant produced in Sf9 Baculovirus
cells is a single, glycosylated polypeptide chain containing 332 amino acids (34-356a.a.)
and having a molecular mass of 36.2kDa. (Molecular size on SDS-PAGE will appear
at approximately 28-40kDa).
IMPAD1 is expressed with a
6 amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
IMPAD1 protein solution (0.5mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Biological Activity
Specific
activity is > 5,000 pmol/min/ug and is defined as the amount of enzyme that
hydrolyze Adenosine 3, 5-diphosphate per minute at pH 7.5 at 25C.
Amino acid sequence
ADPGRFSLFG LGSEPAAGEA
EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK TAFPNVQINT
EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP
VLGVIHKPFSEYTAWAMVDG GSNVKARSSY
NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG
GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGH
HHHH HH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
