Catalogue number
PRO-1751
Synonyms
Receptor-Associated Protein Of The Synapse, 43 KDa
Postsynaptic Protein, Acetylcholine Receptor-Associated 43 KDa Protein, RNF205,
RING Finger Protein 205, rapsyn, Receptor-Associated Protein Of The Synapse,
43Kd, 43 KDa Receptor-Associated Protein Of The Synapse, RAPSYN, RAPsyn, CMS1D,
CMS1E.
Introduction
43
kDa receptor-associated protein of the synapse (RAPSN), is expressed in the postsynaptic
membrane of skeletal muscle. RAPSN is essential for the clustering of nicotinic
acetylcholine receptors (nAChR). In addition RAPSN self-associates via at least
two of its seven tetra-tricopeptide repeats (TPRs). RAPSN interacts with
the large intracellular domain of the nAChR a subunit all the way through the
hydrophobic surface of the coiled-coil domain.
RAPSN modifies trafficking of AChR within the cell. Overexpression
inhibits agrin-induced AChR clustering pathway. RAPSN also plays a role in
postsynaptic congenital myasthenic syndromes.
Description
RAPSN Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 435 amino acids (1-412aa) and having a molecular mass of 48.8kDa.
RAPSN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
RAPSN protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMGQDQTK QQIEKGLQLY QSNQTEKALQ VWMKVLEKGS DLVGRFRVLG
CLVTAHSEMG RYKEMLKFAV VQIDTARGLE DADFLLESYL NLARSNEKLC EFHKTISYCK TCLGLPGTRA
GAQLGGQVSL SMGNAFLGLS LFQKALESFE KALRYAHNND DTMLECRVCC SLGSFYAQVK DYEKALFFPC KAAELVNDYG KGWSLKYRAM
SQYHMAVAYR LLGHLGSAME CCEESMKIAL QHGDRPLQAL CLLCFADIHR SRGDLETAFP RYDSAMSIMT
EIGNRLGQVH VLLGVAKCWM ARKVQDKALD AIEKAQDLAE EVGNKLSQLK LHCLSESIYR SKGLQRELRT HVVRFHECVE ETELYCGLCG ESIGERNSRL QALPCSHIFH
LRCLQNNGTR SCPNCRRSSM KPGFV
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
