Catalogue number
PRO-1316
Synonyms
Heme-binding protein 1, p22HBP, HEBP1, HBP, HEBP.
Introduction
Heme Binding Protein 1 (HEBP1) is an intracellular tetrapyrrole-binding protein. HEBP1 is comprised of a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and stimulates calcium mobilization and chemotaxis in monocytes and dendritic cells.
Description
HEBP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (1-189 a.a.) and having a molecular mass of 23.5kDa.
HEBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
HEBP1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMLGMIKN SLFGSVETWP WQVLSKGDKE EVAYEERACE GGKFATVEVT DKPVDEALRE AMPKVAKYAG GTNDKGIGMG MTVPISFAVF PNEDGSLQKK LKVWFRIPNQ FQSDPPAPSD KSVKIEEREG ITVYSMQFGG YAKEADYVAQ ATRLRAALEG TATYRGDIYF CTGYDPPMKP YGRRNEIWLL KT.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
