Catalogue number
PRO-332
Synonyms
Transducin Beta Chain 3, G Protein, Beta-3 Subunit, GTP-Binding
Regulatory Protein Beta-3 Chain, Guanine Nucleotide-Binding Protein
G(I)/G(S)/G(T) Beta Subunit 3, Guanine Nucleotide-Binding Protein
G(I)/G(S)/G(T) Subunit Beta-3, Hypertension Associated Protein, Guanine
Nucleotide Binding Protein (G Protein), Beta Polypeptide 3, GNB3.
Introduction
GNB3
belongs to the WD repeat G protein beta family. GNB3 is an important regulator
of alpha subunit, as well as of certain signal transduction receptor and effector.
A single-nucleotide polymorphism (C825T) in GNB3 is linked with essential
hypertension and obesity. In addition, this polymorphism is also linked with
the occurrence of the splice variant GNB3-s, which seems to have increased
activity. GNB3-s is an example of alternative splicing caused by a nucleotide
change outside of the splice donor and acceptor sites. Further splice variants
may exist for GNB3, however they have not been fully described. Among the
diseases associated with GNB3 are syncope, and aortic coarctation.
Description
GNB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 363 amino acids (1-340 a.a) and having a molecular mass of 39.6kDa.
GNB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
GNB3 protein solution (0.5mg/ml) containing 20mM Tris-HCl
buffer (pH8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMGEMEQL RQEAEQLKKQ IADARKACAD VTLAELVSGL EVVGRVQMRT RRTLRGHLAK IYAMHWATDS KLLVSASQDG KLIVWDSYTT NKVHAIPLRS SWVMTCAYAP SGNFVACGGL DNMCSIYNLK SREGNVKVSR ELSAHTGYLS CCRFLDDNNI VTSSGDTTCA LWDIETGQQK TVFVGHTGDC MSLAVSPDFN LFISGACDAS AKLWDVREGT CRQTFTGHES DINAICFFPN GEAICTGSDD ASCRLFDLRA DQELICFSHE SIICGITSVA FSLSGRLLFA GYDDFNCNVW DSMKSERVGI LSGHDNRVSC LGVTADGMAV ATGSWDSFLK IWN.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
