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Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119 31-1021-00|产品详情|进口橙子视频旧款采购网




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    上海橙子视频app安卓下载生物科技公司
    Tel:400-968-7988    021-33779008
    Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119
    品牌:RevMAb
    货号:31-1021-00
    规格:100ug
    货期:电询

    商品详情 参考文献 相关资料

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    Product Details
    Product Name Anti-Human IgG3 Rabbit Monoclonal Antibody [RM119]
    Product Description Rabbit monoclonal to Human IgG3
    Catalog No. 31-1021-00
    Clone Name RM119
    Product Data Sheet Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
    Specificity RM119 reacts to the heavy chain of human IgG3, RM119 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
    Immunogen Human IgG3
    Application Immunohistochemistry (IHC)
    Flow Cytometry (FC)
    Immunocytochemistry (ICC)
    ELISA
    Species Reactivity Human
    Conjugate None
    Intended Use Research Use Only
    .
    Properties
    Form Liquid
    Storage Instructions Store at -20.0°C
    Stability Stable for 1 Year at -20.0°C from date of receipt
    Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
    Volume/Item Size 100ug
    Concentration 1.0 mg/mL
    Purity Protein A affinity purified from an animal origin–free culture supernatant
    Clone Type Monoclonal
    Isotype Rabbit IgG
    Usage ELISA: 50ng/well – 200ng/well (for Capture);
    0.05ug/mL – 0.2ug/mL (for Detection);
    Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
    Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
    Keywords
    .
    Product Images
    Detection

    Detection of human IgG3 in monkey serum.
    Sandwich ELISA using RM119 as the capture
    antibody, and biotinylated anti-human light
    chains ( κ+ λ) antibody RM129 as the detection
    antibody, followed by a HRP conjugated streptavidin.

    Sandwich

    Sandwich ELISA using RM119 as the capture antibody
    (100ng/well), and biotinylated anti-human light chains ( κ+ λ)
    antibody RM129 as the detection antibody,
    followed
    by an HRP conjugated streptavidin.

    ELISA

    ELISA showing RM119 reacts only to human IgG3,
    and not to any other IgG subclasses (IgG1, IgG2,
    or IgG4), and no cross reactivity to IgM, IgA, IgD,
    IgE, mouse IgG, rat IgG, or goat IgG.

    A

    A titer ELISA using RM119. The plate was coated
    with different amounts of human IgG3. A serial dilution
    of RM119 was used as the primary antibody, followed
    by an alkaline phosphatase conjugated anti-rabbit IgG
    as the secondary antibody.

    Immunohistochemical staining of formalin fixed
    and paraffin embedded human lymphoid tissue
    section using anti-human IgG3 rabbit monoclonal
    antibody (Clone RM119).

    ELISA

    ELISA showing RM119 does not react to monkey
    IgG. The plate was coated with Rhesus monkey IgG.
    A serial dilution of RM119 and a monkey IgG binding
    antibody (positive control) was used as the detection
    antibody.

    Immunohistochemical staining of FFPE Tonsil tissue
    sections using Anti-Human IgG3 antibody RM119
    at a 1:2000 dilution.

    Image courtesy of Shaomin Hu, MD, PhD,
    Department of Anatomic and Clinical Pathology.
    Montefiore Medical Center
    Albert Einstein College of Medicine.
    Bronx, New York, USA


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