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 monoclonal anti-Glutamate antibody 2D7|产品详情|进口橙子视频旧款采购网




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    monoclonal anti-Glutamate antibody
    品牌:Swant
    货号:2D7
    规格:2D7
    货期:swant

    品牌:Swant 货号:2D7 品名:monoclonal anti-Glutamate antibody 规格:200ul

    商品详情 参考文献 相关资料

    产品介绍

    品名

    monoclonal anti-Glutamate antibody

    货号

    mAB 2D7

    规格

    200 µl

    宿主动物 

    Mouse monoclonal, IgG1

    抗体形式

    Conc. supernat. lyophil. 

    克隆性

    monoclonal

    产品形式

    Lyophilized (no preservatives)

    产品描述

    The antibodies have been purified from ascitic fluid by ammoniumsulfate precipitation and subsequently by FPLC on Protein-A-Superose. BSA has been added to the eluate, and the material has been dialyzed against water and lyophilized.

    产品重构

    - add the lyophilized material (approx. 1 mg) to 100 µl PBS or another buffered saline (add in order indicated) 

    - allow some time for the material to dissolve; mix by swirling gently 

    - remove particulate matter by centrifugation in a microfuge

    - add 100 µl glycerol (final glycerol conc. 50% v/v) and mix well (vortex)

    产品稀释

    Appropriate final dilutions should be determined experimentally. For LM, we strongly suggest to use postembedding staining and to combine the anti-Glu antibody with a silver-enhanced PAP procedure as described in detail by Liu et al.(1989). For EM, we recommend a postembedding immunogold procedure (see: Liu et al., 1989). 

    储存

    Store stock-solution in freezer (-20°C, not -70°C). Mix stock solution well before making aliquots. 10x diluted solutions may be kept in the refrigerator for some time (several weeks) but should be made 10 mM with sodium azide. Volumes of working dilutions (high dilutions) should be calculated such the they are used up the same day. Avoid polystyrene tubes.

    参考文献

    Liu CJ, Grandes P, Matute C, Cuenod M, Streit P: Glutamate-like immunoreactivity revealed in rat olfactory bulb, hippocampus and cerebellum by monoclonal antibody and sensitive staining method. Histochemistry 1989;90(6):427-45  


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