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with 200 µl bidistilled water

Immunohistochemistry: 1:2'000 - 1:5'000 with the avidin-biotin method. On paraformaldehyde (4%) or formalin-fixed tissue. Cryostate sections. 

Immunoblots: 1:1’000 - 1:2'000. 

We recommend that the optimal dilutions be determined by titration experiments

After reconstitution freeze in small aliquots (e.g. 1 µl). For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Naazide). Avoid repeated freezing and thawing

1. Vito, P., Lacana, E. & D'Adamio, L. Science 271, 521-525 (1996). 

2. Tarabykina, S., Læssø Møller, A., Durussel, I., Cox, J. and Berchtold, M.W., J. Biol. Chem. 275, 10514-10518 (2000). 

3. La Cour JM, Moellerup J., Winding P., Tarabykina S., Sehested M. and Berchtold MW, Am. J. Pathol., 163, 81- 89 (2003).