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The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells.

Target	CD15
Reactivity	Human
Host	Mouse
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	X-hapten on lacto-N-fucose pentaosyl lll.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG3
Conjugation	Unconjugated
Specificity	The CD15 antigen is the X-hapten on lacto-N-fucose pentaosyl lll, which is expressed on circulating granulocytes or tissue granulocytes as well as neutrophils and eosinophils. The CD15 antibody recognizes the carbohydrate structure 3-fucosyl-N-acetyl-lactosamine. It specifically labels neutrophils, eosinophils and monoblastoid precursor cells of the myeloid lineage.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	45 kDa
Swiss Prot	P22084
GeneID	2526
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.