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Myc-Tag Antibody is a Mouse Monoclonal antibody against Myc-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Target	Myc-Tag
Host	Mouse
Clonality	Monoclonal
Tested Applications	WB, IF/ICC, IP
Recommended dilutions	WB: 1/2000 - 1/5000, IF/ICC: 1/50 - 1/500, IP: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	A synthetic peptide of Myc-Tag.
Purification	Affinity purified.
Isotype	IgG
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Concentration	> 1 mg/ml
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.