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The monoclonal antibody is directed against the CD24- antigen, which is expressed on virtually all B-cells: early B-cell precursors, pre-pre-B-cells, pre-B-cells, B- cells, intermediate B-cells, mature B-cells and some plasmacytoid cells are positive while plasmacells are negative. The monoclonal antibody reacts with human granulocytes and their precursors (from promyelocytic stage). The monoclonal antibody also reacts with virtually all pre-B-cell lines and Burkitt cell lines, but is only expressed on less then 50% of the B-cell lymphoblastoid cell lines. Non T-ALL, B-cell NHL and 50% of myelomas as well as a subpopulation of AML is found to be positive. Hairy Cell Leukaemia is found to be weakly positive.

Target	CD24
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Glycoproteins purified from human NALM-1 cell line.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG1
Conjugation	FITC
Specificity	The antibody reacts with the CD24 antigen a heavily glycosylated molecule that migrates as a broad band of 35-45 kDa on both reducing and non-reducing SDS gel electrophoresis. CD24 is attached to the cell membrane via a glycosyl phosphatidylinositol (GPI) anchor and is expressed at multiple stages of B cell development, beginning with the bone marrow CD34 positive pro-B cell compartment and continuing through mature surface IgM positive/IgD positive B cells. CD24 is also expressed on the vast majority of B-lineage acute lymphoblastic leukaemias, B cell chronic lymphocytic leukaemias and B cell non-Hodgkin's lymphoma. In the normal spleen, mantle zone lymphocytes are CD24 strongly positive, but germinal center cells are CD24 negative or CD24 weakly positive.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	35-45 kDa
Swiss Prot	P25063
GeneID	100133941
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.