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Green Fluorescent Protein (GFP) Antibody is a Mouse Monoclonal antibody against GFP. GFP, originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Target	Green Fluorescent Protein (GFP)
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	WB
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Purification	Purified Mouse Monoclonal Antibody.
Isotype	IgG1
Conjugation	Unconjugated
Specificity	Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Buffer	PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.