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CD43, the glycoprotein leukosialin (95-115kDa MW) antigen present on all T lymphocytes and on granulocytes, monocytes and platelets in peripheral blood. The antigen is also expressed cytoplasmically by macrophages. It is also present on all immature hematopoietic cells in the bone marrow. CD43 may be involved in the regulation of B, T and NK cell function.

Target	CD43
Reactivity	Human
Host	Mouse
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Native purified from myeloblastic KG1 Cells.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG1
Conjugation	Unconjugated
Specificity	CD43 is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	95-115 kDa
Swiss Prot	P16150
GeneID	6693
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 2-3 weeks.
Note	This product is for research use only.