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C-RAF Antibody detects endogenous levels of total C-RAF. Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn

Target	C-RAF
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB
Recommended dilutions	WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	A synthesized peptide derived from human C-RAF.
Purification	Immunogen affinity purified.
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	74 kDa
Swiss Prot	P04049
Enzyme Commission Number	EC 2.7.11.1
Buffer	Rabbit IgG in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.