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The 581 antibody reacts with human CD34, an ~105 - 120 kDa type 1 transmembrane glycoprotein expressed on the surface of most human hematopoietic stem and progenitor cells (HSPCs) as well as on mesenchymal stem cells, embryonic fibroblasts, endothelial cells, neurons, and some tumor cell lines. CD34 is expressed only transiently during hematopoiesis, so the frequency of CD34+ cells is low in bone marrow or cord blood (~1 - 5%) and very low (~0.1 - 0.5%) in peripheral blood. CD34 is a marker used to identify and isolate HSPCs capable of cell engraftment. CD34 is thought to mediate attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells during early hematopoiesis, and to be involved in lymphocyte recruitment through binding to the ligands L- and E-selectin. Distinct epitope groups have been assigned to CD34 based on their sensitivity to enzymatic cleavage, with the 581 antibody recognizing a class III epitope (resistant to neuraminidase and O-glycoprotease).

Target	CD34
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Human CD34+ leukemic cells.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG1
Conjugation	APC
Specificity	The CD34 antigen is a single-chain transmembrane glycoprotein, Mr 105 to 120 kilodaltons(kd). The antigen is associated with human hematopoietic progenitor cells and is a differentiation stage–specific leucocyte antigen.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	105 to 120 kDa
Swiss Prot	P28906
GeneID	947
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.