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Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of protease inhibitors. It is highly expressed in embryonic cells and ***** hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium-dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins, Recombinant Human Fetuin A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. The calculated molecular weight of Recombinant Human Fetuin A is 32.9 kDa. Due to glycosylation, recombinant Fetuin A migrates at an apparent molecular weight of 45–55 kDa by SDS-PAGE run under non-reducing conditions.

Source: HEK293 cells

Synonyms: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotien

AA Sequence: A-Chain: APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL

B-Chain: TVVQPSVG AAAGPVVPPC PGRIRHFKV

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit Cathespin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (RLUs). The expected IC₅₀ is ≤ 100nM.