West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDaprotein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
The E.Coli derived recombinant protein contains the West-Nile N-terminus Envelope Virus immunodominant regions. The protein is fused with 6xHis tag. Mw~42kDa. (1mg/ml) 20mM Phosphate buffer pH 7.5. Antigen in ELISA and Western blots, excellent antigen for detection of West-Nile virus with minimal specificity problems. Purified by proprietary chromatographic technique. Immunoreactive with sera of West Nile virus infected individuals.
Application: ELISA Western blot and Lateral flow.