Short chain dehydrogenase/reductase family 16C, member5 (SDR16C5) is active in the oxidative direction as well as in the reductive one. SDR16C5 oxidizes all-trans-retinol in all-trans-retinaldehyde. No activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH.
SDR16C5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (32-269 a.a.) and having a molecular mass of 28.3kDa. SDR16C5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SDR16C5 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.