Introduction: HEXIM1 expression is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. HEXIM1 is a transcriptional regulator which acts as a universal RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex inhibits RNA polymerase II phosphorylation following transcriptional elongation. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
HEXIM1 may play a role in cell cycle progression and tumorigenesis.
HEXIM1 Human Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 396 amino acids (1-359a.a.) and having a molecular mass of 44.8 kDa. HEXIM1 is fused to a 37 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques.
Appearance: Sterile filtered colorless solution.
Formulation: HEXIM1 solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 0.1M NaCl and 10% glycerol.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.