NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
Description: NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 304 amino acids and c-terminal 13 amino acid TAT peptide having a molecular mass of 36.1kDa.
The NANOG is purified by proprietary chromatographic techniques.
Physical Appearance: Sterile Filtered solution.
Formulation: NANOG protein solution contains PBS and 50mM Arginine.
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.