A fully validated, highly specific, affinity purified rabbit polyclonal antibody to GFP, EGFP, PA-GFP, CFP and YFP

Background: The green fluorescent protein (GFP) is composed of 238 amino acid residues (26.9kDa) and exhibits bright green fluorescence when exposed to ultraviolet blue light. It has become a well established reporter for gene expression and marker for protein targeting. Subtle modifications have been made to the sequence of GFP improving its solubility and photostablility (eGFP) to allow acquisition of more complex data (FRET, FRAP). Moreover, subtle residue modifications have been engineered to alter the emission wavelength such as in CFP, YFP to facilitate duel labelling. Anti-GFP Ab is a polyclonal antibody raised in rabbit by immunisation with, and affinity purification against full length GFP (Faix et al., 2001). It produces exceptional staining for GFP, PA-GFP, EGFP, CFP and YFP in western blotting at a dilution of 1:10,000 and bioimaging applications at high dilutions, it has featured in an impressive number of publications.

Tested Applications: Western blot (1:10,000 dilution), IF Microscopy (1:500 - 5000 dilution).