The new CAPTUREome^TM S-Palmitoylation Mini Kit from Badrilla represents an affordable gateway into the emerging field of palmitoylation research. The Mini Kit, based on acyl RAC (resin assisted capture) technology, enables rapid determination of protein palmitoylation status from as little as 1mg of total cell lysate protein.

Kit highlights include

• Competitively priced entry level kit.
• Enables quick and easy capture of S-palmitoylated proteins from as little as 1mg of total cell lysate protein.
• Kit designed to process 2 x 3 biological samples with paired negative controls (i.e. 12 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.

References

1. Forrester M.T., et al. (2011) Site-specific analysis of protein S-acylation by resin assisted capture. J. Lipid Res. 52: 393-398
2. Howie, J. et al (2014) Substrate recognition by the cell surface palmitoyl transferase DHHC5. PNAS 111: 17534–17539