Intended use

The Euro Diagnostica vasopressin kit contains reagents and instructions for the quantitative measurement of vasopressin in plasma or urine. After solid phase extraction (SPE) or ethanol extraction the plasma vasopressin concentrations are measured by radioimmunoassay (RIA). Urine vasopressin concentrations can be measured directly.

For professional use within a laboratory.

This product is also available as research use only. Product code: RB 319 RUO

For complete product description, claims and use, please refer to the Instructions For Use. Contact your local representative for availability in your country.

Background

Vasopressin, or Antidiuretic Hormone (ADH) is a cyclic nanopeptide with a molecular weight of 1083. Its structure is very similar to that of oxytocin, differing in only two amino acids. Endogenous ADH has antidiuretic and pressor activity, both approaching 400 units per mg, with an antidiuretic-to-vasopressin ratio of 1, and a biphasic plasma half-life of 2.5 and 14.5 minutes. ADH is synthesized in the hypothalamic supraoptic nucleus and paraventricular nucleus of primates and transported via exonal flow to the posterior pituitary for storage and eventual release. The clinical application of a vasopressin radioimmunoassay is in diabetes insipidus, psychogenic water intoxication, hyponatraemia, stress conditions, ADH as a neurotransmitter and hypertension studies. ADH values can be influenced by cigarettes, tea, coffee, alcohol and some drugs.

Technical information

After solid phase extraction (SPE) or ethanol extraction of the plasma samples, vasopressin is assayed by a competitive radioimmunoassay. Urinary vasopressin can be measured directly. This assay uses a rabbit anti-vasopressin antiserum and a radioiodinated vasopressin [¹²⁵I] tracer. Bound and free phases are separated by a second antibody bound to solid phase particles, followed by a centrifugation step. The radioactivity in the bound fractions is measured and a typical standard curve can be generated. The values of the extracted samples are corrected for extraction recovery.

Kit components and storage of reagents

The reagents provided in each kit are sufficient for 100 tubes.

Content of the kit

1. Anti-vasopressin  (Reagent A), Nr. of Vials: 1, Containing: Lyophilized anti-vasopressin (Rabbit) for 100 tubes. Colour: Yellow.
2. ¹²⁵I-vasopressin (Reagent B), Nr. of Vials: 1, Containing: 28 KBq or 0.75 μCi. Lyophilized. Specific activity: 62-77 MBq/nmol (1700-2100 μCi/nmol). Colour: Blue.
3. Double antibody solid phase (Reagent C), Nr. of Vials: 1, Containing: Goat anti-rabbit IgG’s bound to solid phase. 11 mL suspension.
4. Assay buffer (Reagent D), Nr. of Vials: 2, Containing: 0.05 M phosphate buffer with 0.25% HSA, 0.25% EDTA disodium salt, 0.05% NaN₃, 500 KIU Trasylol/mL. 2 x 50 mL (liquid).
5. Vasopressin standard 60 pmol/L (Reagent E), Nr. of Vials: 1, Containing: 5.00 mL vasopressin standard, 60 pmol/L. Lyophilized in assay buffer.
6. Vasopressin, low control (Reagent F), Nr. of Vials: 1, Containing: 2.00 mL lyophilized vasopressin control, low level.
7. Vasopressin, high control (Reagent G), Nr. of Vials: 1, Containing: 2.00 mL lyophilized vasopressin control, high level.

Reagent preparation and storage

This kit is stable until the stated expiry date if stored as specified.

Upon receipt of the kit, all reagents should be stored at 2-8° C.

The reconstituted reagents should be stored according to table on page 7.

The reconstituted reagents are stable according to table on page 7, but no longer than to the expiry date.