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CHD8+/− iPSC

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CHD8+/− iPSC

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	Print Version
Safety	Biosesafety Level II
Organism	Human
Source Organ	Skin
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Description	CHD8 has a role in chromatin dynamic, transcription and cell survival in which is interacts with p53 to regulate cell cycle genes. It has been associated with developmental disorders like ASD (autism spectrum disorders). CHD8+/− iPSC were derived from skin fibroblasts of patients with 22q11.2 deletion and diagnosis of a psychotic disorder in which CRISPR/Cas9-mediated CHD8 knockout was performed. It may be utilized for studies in development. Growth Properties: Adherent Morphology: Polygonal
Concentration	10⁶cells/vial
Pluripotency	1) Pluripotency was confirmed by immunocytochemistry for iPSC markers Tra-1-60, Tra-1-81, SSEA3, and SSEA4; 2) Western blots used to identify the knockout of CHD8
Propagation	Matrigel (BD) coating is required for cell adhesion to the culture vessels. Grow cells in Matrigel-coated culture vessels with the following conditions. The base medium for this cell line is mTeSR1 (Stem Cell Technologies). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.