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REF-iPSC

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REF-iPSC

商品详情参考文献相关资料

	Print Version
Safety	Biosesafety Level II
Organism	Rat
Source Organ	Embryo
Concentration	10⁶cells/vial
Propagation	The optimized medium for this cell line is Rat iPSC Culture Medium available from ABM (TM021). This cell line needs to be cultured on inactivated REF feeder cells (T2017). Atmosphere:air: 95%, CO₂: 5%; Temperature: 37.0°C.
Subculturing	1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new feeder cell culture vessel. 6. Incubate cultures at 37°C. Be sure to use inactivated REF feeder cells (T2017).
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.