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Transdifferentiated Mesenchymal Stem Cells

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Transdifferentiated Mesenchymal Stem Cells

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	Print Version
Safety	Biosesafety Level II
Organism	Human
Source Organ	Cord Blood
Concentration	10⁶cells/vial
Induction	tMSC was generated using lentiviral transduction of Human Cord Blood CD34+ cells.
Pluripotency	Positive for markers by immunofluorescence and flow cytometry. tMSC is capable of differentiating into osteocytes, chondrocytes and adipocytes in vitro. tMSC implantation into immunodeficient mice can form bone, tendon, cartilage and skeletal muscles.
Propagation	The base medium for this cell line is the MSC medium available from ABM (Cat.No. TM022). To make the complete growth medium, add the growth supplements prior to use. Addition of CHIR99021 (Cat. No. G611) to the medium along with culturing the cells on fibronectin-precoated non-TC well plates under hypoxia (3-5%) is recommended to obtain maximal cell proliferation. If the cells are cultured in normoxia (20% O2), the transdifferentiated MSCs may not be able to expand extensively. Temperature: 37.0°C.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.