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MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells

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MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells

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	Print Version
Safety	Biosesafety Level II
Organism	Rat
Source Organ	Embryo
Growth Properties	Adherent
Morphology	Fibroblast-like
Applications	For Research Use Only. The inactivated REFs have been tested to be significantly better than inactivated MEF in supporting human iPSC reprogramming and long-term undifferentiated proliferation of human iPS cells and human ground state naive pluripotent stem cells. There is no need to change medium at weekend if human iPS or ES cells are split on Friday and cultured on this REF feeder line in iPSC Culture Medium (TM020).
Propagation	The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 15%; 0.1 mM Non-Essential Amino Acids; and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.