Warning: mkdir(): No space left on device in /www/wwwroot/zhenghe1718.com/func.php on line 127

Warning: file_put_contents(./cachefile_yuan/pkscc.com/cache/cd/99041/50922.html): failed to open stream: No such file or directory in /www/wwwroot/zhenghe1718.com/func.php on line 115
 Enhanced-Primary-Human-Hepatocytes-\u2013-1-Million |产品详情|进口橙子视频旧款采购网




  • 橙子视频app安卓下载,91橙子视频,橙子视频旧款,橙子视频在线官网

    订购信息
    上海橙子视频app安卓下载生物科技公司
    Tel:400-968-7988    021-33779008
    Enhanced-Primary-Human-Hepatocytes-\u2013-1-Million
    品牌:Abmgood
    货号:
    规格:
    货期:

    Enhanced-Primary-Human-Hepatocytes-\u2013-1-Million

    商品详情 参考文献 相关资料
    Print Version
    Description Enhanced Primary Human Hepatocytes are expanded primary cells which retains the physiologically relevant profile and phenotype of primary hepatocytes. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for metabolism studies. The late expansion cells are tested for CYP induction and inhibition. It is not recommended for further extensive passaging after thawing but can be used for long term culture which is necessary for cell-based assays.

    Enhanced Primary Human Hepatocytes -1 Million is a lower cell count of our expanded primary hepatocytes offered at abm. We have a higher cell count available as the Enhanced Primary Human Hepatocytes – 5 Million (Cat. T5996).
    Procedure Overview
    Biosesafety Level II
    Organism Homo sapiens
    Source Organ Liver
    Growth Properties Adherent
    Morphology Polygonal
    Image
    Image
    Image
    Image
    Recommended Seeding Density 10,000 cells/cm2
    Applications For Research Use Only
    Propagation Use of Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. We strongly recommend end users to purchase the Applied Cell Extracellular Matrix (G422) to coat your cell culture vessels. Use the ready-to-use Hepatocyte Thawing Medium (TM102) and the Enhanced Primary Human Hepatocytes Media Kit (TM103) which comes with the Hepatocyte Growth Basal Medium, and Hepatocyte Growth Supplement Mix available from abm . Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.

    To make complete Hepatocyte Growth Basal Medium add the entire content of Hepatocyte Growth Supplement Mix into Hepatocyte Growth Basal Medium and mix properly. Addition of the Hepatocyte Growth Supplement Mix may change medium appearance more opaque.

    To thaw:

    1. Pre-warm Hepatocyte Thawing Medium and fully supplemented Hepatocyte Growth Medium to room temperature.

    2. Carefully remove cryovial from storage tank.

    3. Thaw cells in 37°C water bath until only a small chunk of ice is left. Do not shake the vial, or take it out of the water during thawing, as this will damage the cells.

    4. Spray the vial and the tube containing 50 ml of thawing medium with 70% ethanol and transfer to a Biosesafety cabinet.

    5. Transfer the now completely thawed cell suspension from the cryovial into 50 ml Hepatocyte Thawing Medium by gently pouring the cells into the medium.

    6. Use a 1 ml pipette to transfer 1 ml of the thawing medium back to the cryovial and pour the contents back into the 50 ml tube. Repeat this process twice to completely remove the cells from the cryovial.

    7. Pellet the cells by centrifuging at 90×g for 5 min at RT. Important note: Higher g-forces will significantly reduce cell recovery.

    8. Aspirate the supernatant without disturbing the pellet. Leave approximately 200-400 μl medium on top of the cells. 9. Gently loosen and re-suspend the cells without adding any extra medium by agitating and rotating the tube. Do not vortex or shake the cells as this will compromise cell survival.

    10. Add an appropriate volume of pre-warmed supplemented Hepatocyte Growth Medium to the pellet (~1ml per million cells thawed) and re-suspend the cells. Avoid pipetting the cells up and down.

    11. Determine viable cell number by cell count.

    12. Dilute hepatocytes in pre-warmed, fully supplemented Hepatocyte Growth Medium and seed at ~10,000 cells/cm2 in collagen coated cell culture flasks (e.g. T175) or appropriate cell culture dishes.

    13. Incubate the cells at 95% humidity, 37°C and 5% CO2.

    Subculturing To Subculture:

    1. Pre-warm PBS, trypsin/EDTA and Hepatocyte Medium to 37°C.

    2. Carefully aspirate the culture supernatant.

    3. Wash the plate once with ~100 µl PBS/cm2.

    4. Add ~50 µl/cm2 trypsin/EDTA (0.05%/0.02% EDTA).

    5. Incubate for 3-4 min at 37°C until most of the cells are rounded up and detached. Avoid incubating the cells for more than to 10 min.

    6. Gently tap the cell culture vessel to detach remaining adherent cells.

    7. Stop the trypsin activity by adding twice the volume (100 µl/cm2) of supplemented Hepatocyte Growth Medium containing 10% FBS (TM999) or Trypsin Neutralization Solution (Lonza).

    8. Rinse the surface with the cell suspension using a pipette.

    9. Transfer the complete suspension to a tube and centrifuge at 90xg for 5 min at RT.

    10. Aspirate the supernatant without disturbing the pellet. Leave approximately 200-400 μl medium on top of the cells. 11. Gently loosen and re-suspend the cells without adding any extra medium by agitating and rotating the tube. Do not vortex or shake the cells as this will compromise cell survival.

    12. Add an appropriate volume of pre-warmed supplemented Hepatocyte Growth Medium to the pellet (~1ml per million cells thawed) and re-suspend the cells. Avoid pipetting the cells up and down.

    13. Determine viable cell number by cell count.

    14. Dilute hepatocytes in pre-warmed, fully supplemented Hepatocyte Growth Medium and seed at ~10,000 cells/cm2 in collagen coated cell culture flasks (e.g. T175) or appropriate cell culture dishes. We strongly recommend end users to purchase the Applied Cell Extracellular Matrix (G422) to coat your cell culture vessels.

    15. Incubate the cells at 95% humidity, 37°C and 5% CO2.
    Preservation We do not recommend freezing down the Enhanced Primary Human Hepatocytes.

    Quality Control Each lot has been tested for:
    1. >90% plateability
    2. Post-thaw viability
    3. CMV, HIV, HBV, HCV, and Mycoplasma negative
    4. Hepatic markers via immunofluorescence staining: CK8+, AAT+, CK18+, HAS+, and AFP-
    5. Basal and inducible CYP activities
    6. Glycogen storage via PAS staining
    Disclaimer 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.
    2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evalsuate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical locations. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
    3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
    4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
    • 关于橙子视频app安卓下载
    • 购物流程
    • 支付方式
    • 配送方式
    Copyright@ 2003-2026  进口橙子视频旧款采购网版权所有     

    BIOLEAF热搜   BIOLEAF91橙子视频   BIOLEAF ELISA   BIOLEAF橙子视频旧款   BIOLEAF品牌   BIOLEAF抗体   BIOLEAF耗材   BIOLEAF小仪器

    sitemap   细胞库查询   危险品图标

    本公司网站所展示销售的产品仅供科研!

             沪ICP备08023583号-6     
    产品咨询
    QQ扫码沟通
    在线客服
    服务电话
    400-968-7988
    扫码关注
    微信公众号二维码

    沪公网安备 31011202007338号

    网站地图