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Phi29 DNA Polymerase

品牌：Abmgood

货号：E014

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Phi29 DNA Polymerase

商品详情参考文献相关资料

	Print Version
Description	Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.
Application	• Rolling circle amplification (RCA) • Multiple displacement amplification (MDA) • Whole genome amplification (WGA) • DNA template preparation for sequencing • Protein-primed DNA amplification
Form	Enzyme supplied with 5X Reaction Buffer
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Concentration	10 U/μl
Storage	Store all components at -20°C.
Storage Buffer	50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol.
Notes	One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use .