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CHOPWT9 iPSC

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CHOPWT9 iPSC

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	Print Version
Safety	Biosesafety Level II
Organism	Human
Donor	Female
Source Organ	Peripheral Blood Mononuclear Cells
Growth Properties	Adherent
Morphology	Polygonal
Markers	Oct4, Sox2, Nanog, Rex1, DMNT3B, and ABCG2
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Description	The CHOPWT9 IPSCs were established by Sendai viral infection carrying reprogramming factors Oct3/4, Sox2, c-Myc, and Klf-4 of peripheral blood mononuclear cells collected from a healthy ***** female. These cells are capable of in vivo tetratoma formation as tested on NOD/SCID mice. The CHOPWT9 IPSCs are valuable in the field of developmental biology as they are useful as a control for applications such as differentiation analyses to the three germ layers and derivative tissues.
Quality Control	1) Flow cytometry analysis of pluripotency markers; 2) Karyotype analysis of chromosomal G-band; 3) qPCR of pluripotency genes
Concentration	10⁶cells/vial
Propagation	The iPSCs requires a co-culture with mouse embryonic fibroblasts (MEFs) to proliferate. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: KnockOut^TM Serum Replacement (Invitrogen; A3181502) to final concentration of 15%, 1% NEAA (Invitrogen; 11140050), 1% L-glutamine (G275), 0.1 mM 2-mercaptoethanol (Invitrogen; 21103-049) and 1% Penicillin/Streptomycin Solution (G255). Filter medium with 0.45 µM, before adding 5-10 ng/ml human bFGF (Z101455).Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. Add 10 µM ROCK inhibitor (R&D Systems; 1254/50) to media when plating iPSCs on MEFs.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.